Using software breakpoints impacts the checksum result.
The debugger inserts a dedicated “software breakpoint” instruction on addresses where software breakpoints are set. These “software breakpoint” instructions are not visible, e.g. in the Disassembly or Memory Window at software breakpoints addresses. When a software breakpoint is removed, the original instruction is restored in its place.
When performing any kind of checksum in your application, remove all software breakpoints.
Default hardware breakpoints do not impact the checksum result.